Ensuring Data Quality Through Mass Balance Calculations in Culture's Bioreactors

In order to make improvements to a cell line and the cell culture process, it is important to have a thorough understanding of carbon fluxes within a bioreactor.While volumetric measurements can be informational, such as titer in g/L, they do not provide information around the total amount of product made or total loss of carbon to byproducts such as CO2. Without calculation of mass balances and use of mass-based measurements, it is difficult to gain more than a cursory estimate of clone performance and product fluxes. Measurements taken throughout a batch to accurately track total mass additions and losses enable the calculation of mass-based metrics from the batch (or run?). Culture Biosciences’ automated cloud bioreactors are equipped with hardware and software features that allow for accurate tracking of mass (both liquid and gas), which provides a more comprehensive assessment of cell line performance and opportunities for both clone and process improvements.